HLA Testing
| Property | High-resolution sequencing | Single-allele assay |
|---|---|---|
| Method | PCR sequencing of Exon 2 and 3 for class I HLA | PCR with allele-specific primers |
| Resolution | Minimum 4 digit (i.e. 2 field) | Presence or Absence |
| Zygosity | Yes. Can be determined (i.e. homozygous vs heterozygous) | No |
| Use Case | Transplant typing, research | Pharmacogenomics (e.g.HLA-B*58:01 and allopurinol) |
| Cost/Time | Higher | Lower |
References
1.
Madden K, Chabot-Richards D. HLA testing in the molecular diagnostic laboratory. Virchows Archiv. 2019;474(2):139-147. doi:10.1007/s00428-018-2501-3
2.
Rwandamuriye FX, Chopra A, Konvinse KC, et al. A rapid allele-specific assay for HLA-a*32:01 to identify patients at risk for vancomycin-induced drug reaction with eosinophilia and systemic symptoms. The Journal of Molecular Diagnostics. 2019;21(5):782-789. doi:10.1016/j.jmoldx.2019.04.006