Tryptase

Figure 1: Tryptase Gene

Figure 2: Tryptase Secretion

Figure 3: Tryptase Secretion

Figure 4: Acute and Baseline Tryptase

Tryptase is a natural serine protease

average tryptase, average tryptase HAT, average tryptase ISM, average tryptase ASM

Elevated basal tryptase >= 11.5 ng/mL Can get basal tryptase at 24 hrs after anaphylaxis event

Anaphylaxis >= 20% increase from basal tryptase + 2 ng/mL

TPSAB1 increased: HaT, SM CNV (alpha tryptase) alpha or beta tryptase constitutively released (measure of MC burden)

TPSB2 Beta preformed granule release during anaphylaxis/severe MC activation

Total Tryptase = a + B by ImmunoCAP (R) In vitro assay half-life ~90 minutes peak at 1 hr stored primarily in MC